KMID : 0380020170320040311
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Korean Journal of Biotechnology and Bioengineering 2017 Volume.32 No. 4 p.311 ~ p.318
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Study of Anti-atopic Dermatitis Effects of Juice of Raphanus sativus var in HaCaT Cell Line
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Lee Seung-Je
Lim Eun-Gyeong Kim Ga-Yeon Jeon Mi-Ji Kim Sang-Yong Kim Young-Min Yoo Je-Geun
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Abstract
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Raphanus sativus var is widely known as herbs for reducing skin heat and for deciphering internal organs. Also, Raphanus sativus var has various medical effects. But, the effects of atopic treatment of Raphanus sativus var were not investigated. Therefore, we assessed the anti-atopic dermatitis and anti-inflammatory effects of juice of Raphanus sativus var in HaCaT cells. MTT assay showed that the juice of Raphanus sativus var was not cytotoxic effect on the HaCaT cells. The HaCaT cell migration was demonstrated by cells migration assay. TNF-¥á and IFN-¥ã induces production of inflammatory cytokines such as CCL17, CCL 27, IL-1¥â, IL-6 and IL-8 expression. Juice of Raphanus sativus var could inhibit TNF-¥á and IFN-¥ã induced mRNA expression levels of CCL17, CCL 27, IL-1¥â, IL-6 and IL-8 gene. Furthermore, we determined the effects of skin turnover through organotypic cell culture and H&E stain. Our study showed that the treatment of HaCaT cells with the juice of Raphanus sativus var in the observed that its skin recovered to normal condition. These results suggested that the possibility Juice of Raphanus sativus var for prevention and treatment of skin inflammatory diseases such as atopic dermatitis.
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KEYWORD
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HaCaT cells, Raphanus sativus var, atopic dermatitis, anti-inflammatory
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